88: SULFOLOBUS MEDIUM

Modifications: Use only 0.50 g/l yeast extract and supplement medium with 10.00 g/l powdered sulfur. Sterilize sulfur separately by steaming for 3 hours on each of 3 successive days (see medium 35) and add aseptically to the autoclaved medium. Adjust the pH of the final medium to 2.5.

Growth observed: (+)

Growth conditions: 2-3 days 65 °C